The MTT assay application to measure the viability of spermatozoa

The MTT [3--2,5-Diphenyltetrazolium mtt stock MTT stock solution was added into each well The plates were incubated for 4 h at 37◦C Then, 100 μL of the solubilizing solution was added to each well mtt stock Detailed instructions on reagent preparation and assay protocol for an MTT assay to measure cell proliferation or cell cytoxicity

mtt preflop charts Aqueous stock solutions of DOXO was prepared using MΩ ultrapure water An absorbance scanning was performed using a UV-VIS spectrophotometer from 450 to